Resources:

Labs

Hearst Memorial Mining Building

The Biomimetic Materials and Tissue Engineering Laboratory is located in the newly renovated Hearst Memorial Mining Building. The laboratory has the capability to efficiently synthesize and characterize the physical, chemical, and surface properties of materials, and then examine mammalian cell response to these biomaterials under clean room conditions. The laboratory has approximately 2,000 sq.ft. of general wet chemistry laboratory space upon which both major and minor equipment is located, including polymer synthesis and processing facilities, static light scattering, HPLC with UV/radiation detectors, surface characterization equipment, rheology equipment, optical microscopy and digital image acquisition and processing, and cell culture, molecular biology (i.e. PCR), and tissue harvesting facilities. In addition, central facilities within UC Berkeley and Lawrence Berkeley National Laboratory (LBNL) provide additional resources for advanced surface analysis using XPS and AFM.

Facilities include:

• Surface chemistry laboratory: Positive pressure room dedicated to surface modification of biomaterials. Equipment includes a laminar flow hood, a fume hood with a spinner, nitrogen glove box, oxygen plasma, drying oven, QCMD, sonicator, light box, microbalance, contact angle measurement system, UV light table, UV chamber reactor, nitrogen filled sample storage box.
• Tissue culture room: Positive pressure room dedicated to cell culture. Equipment includes a dedicated refrigerator and freezer, liquid nitrogen dewar, one incubator for general cell experiments, a dedicated human stem cell incubator, laminar flow hoods, a dedicated biosafety cabinet, a second laminar flow hood, a light microscope, centrifuge.

Stanley Bioscience and Bioengineering Facility

The new facilities in Stanley Hall, funded by QB3, currently house our wet chemistry lab and tissue culture for human embryonic stem cells. This includes:

• General wet chemistry laboratory: cuvette/multiwell plate spectrophotometer and fluorimeter, HPLC, rotovap, lyophilizer, centrifuge, in situ PCR machine, gel boxes and power supply for horizontal and vertical electrophoresis, a digital camera/lightbox for imaging ethidium bromide stained gels, as well as blotting apparatus and an incubator/shaker with a capacity of up to 5 liters, rheometer.
• Tissue culture room: Positive pressure room dedicated to cell culture. Equipment includes dedicated refrigerators and freezer, on-site liquid nitrogen supply, incubators for general cell experiments, dedicated human stem cell incubators, dedicated biosafety cabinets, centrifuge.
• Digital imaging microscope and darkroom: vibration isolation table (TMC), inverted fluorescence/phase contrast microscope with an automated X-Y-Z stage controller (Nikon), black and white CCD camera and controller (Q-imaging), video camera (Dage), image processing software (IPLab), Macintosh computer equipped with 21” high-resolution color monitors for digital acquisition and processing of images, 12” high resolution B/W monitor (Sony), 200 W mercury lamp and housing (Oriel).